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LC-MS/MS法研究荷叶生物碱在大鼠体内的组织分布
LC-MS/MS法研究荷叶生物碱在大鼠体内的组织分布
叶林虎1,2,孔令提1,闫明珠1,曹芳瑞1,常 琪1

1 中国医学科学院北京协和医学院药用植物研究所,北京 100193; 2 贵州省毕节市第一人民医院,毕节 551700
Tissue distribution of the lotus alkaloids in rats determined by LC-MS/MS
(1 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; 2 Department of Pharmacy,the First People's Hospital of Bijie, Bijie 551700, China)

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起始页:1503

摘要:[摘要] 目的: 建立荷叶碱(NF)、氮-去甲荷叶碱(N-NF)和2-羟基-1-甲氧基阿朴啡(HMA)的LC-MS/MS分析方法,对其在大鼠的组织分布进行研究。方法: 通过给大鼠静脉注射荷叶总生物碱10 mg?kg-1后,采用LC-MS/MS方法测定各组织中主要生物碱成分NF,N-NF和HMA的含量。结果:给大鼠静脉注射总生物碱后,3个主要生物碱成分NF,N-NF和HMA能迅速(5 min)分布到各组织,其中在肝脏、肺、脾脏和肾脏等组织中具有较高的浓度,6 h后显著下降。结论: 建立的方法简便易行、准确可靠,可用于大鼠体内NF,N-NF 和HMA的组织分布研究;NF,N-NF和HMA在大鼠体内分布快且广泛,并在体内维持较长时间。

关键词:[关键词] 组织分布; LC-MS/MS; 荷叶碱;氮-去甲荷叶碱;2-羟基-1-甲氧基阿朴啡

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Abstract:[Abstract] Objective: To establish an LC-MS/MS method for the analysis of tissue distribution of nuciferine (NF), N-nornuciferine (N-NF) and 2-hydroxy-1-methoxyaporphine (HMA) in rats. Methods: Total alkaloids were administrated intravenously (10 mg?kg-1) to rats and the concentrations of NF, N-NF, and HMA in biological samples were determined with established LC-MS/MS method. Results: The results showed that NF, N-NF and HMA could be quickly (within 5 min) distributed to various tissues, especially in the liver, lung, spleen and kidney with relative high concentrations, and decreased significantly in 6 hours in most tissues. Conclusion: The established method is simple and accurate, and can be used to study the tissue distribution of NF, N-NF and HMA in rats. The NF, N-NF and HMA are widely distributed and remained in the bodies for a relative long period.

Key words:[Key words] tissue distribution; LC-MS/MS; nuciferine; N-nornuciferine; 2-hydroxy-1-methoxyaporphine



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